Favour to get me out of a potential disaster

[cj]

Hi, could anyone out there with a uni connection possibly get hold of a paper for me, our institutional subscription to PNAS apparrently ran out on monday and I'm supposed to have read it by tomorrow!

It's in Proceedings of the National Acadamy of Sciences and if your uni subscribes I think it will recognise the IP address when you bring the website up (http://www.pnas.org/) The paper is called

Inactivation of a Pseudomonas aeruginosa quorum-sensing signal by human airway epithelia

Carlene K. Chun, Egon A. Ozer , Michael J. Welsh , Joseph Zabner and E. P. Greenberg

and is in the current issue so linked from the front page as 'Mammalian cells inactivate bacterial quorum sensing'

If anyone could do this it would be ace, my email is christine.jones@st-hughs.ox.ac.uk

[Becks]

Whooo! I wish I did Biochem

[cj]

Beats plants any day though

[Braddie]

Seems that the Business School deems Biological research papers inappropriate reading matter......
Why????
Anway - back to C++ and some lovely derivative pricing

[ic]

you have it in the post, cj

[Ali]

Wow, you have such exciting reading material cj...i don't understand half the words in the title!! Am only a simple geographer though....

[Nailest]

Did all the volumes run out of the RSL as well?

Let me know if you still need it CJ. Is this for Judy Armitage?

Although having now seen that you posted that yesterday, and tomorrow hence meant today, my offer's probably a bit redundant. Not that it wasn't redundant already, but what the hey.

[samsonite]

aw man, when i saw this post coem up last night i clicked straight in to take the piss, but then felt that maybe i should stop being a nasty bastard and not make your bad situation worse, and now others have got in ahead of me and i feel cheated......doh!

[brooner]

try reading the damn thing first, there's no need to take the piss of anyone who can get through the title, let alone the following 4 pages...

Materials and Methods
Epithelial Cell Cultures. Primary cultures of human airway epithelia
were grown on permeable supports at the air–liquid interface
as described (27). Epithelia were used after at least 14 days of
culture when they had developed morphologic and functional
properties of airway epithelia (27).
Other cell lines were grown as monolayers in 24-well dishes.
These cell lines were A549 cells, established from a human
bronchoalveolar carcinoma and grown in DMEM_Ham’s F-12
medium (1:1) with 10% FCS and 1% L-glutamine; CaCo-2 from
a human colon carcinoma grown in Eagle’s minimal essential
medium (EMEM), 20% FCS, and 1% nonessential amino acids
(NEAA); Madin–Darby canine kidney (MDCK) cells from
canine kidney grown inDMEM_Ham’s F-12 (1:1) and 10% FCS;
CHOfrom Chinese hamster ovary grown in Ham’s F-12 and 10%
FCS; HeLa from human cervix cultured in EMEM, 10% FCS,
and 1% NEAA; 293T from human embryonic kidney and COS-7
from monkey kidney, both grown in DMEM and 10% FCS; and
primary cultures of human lung fibroblasts (HLF) grown in
DMEM and 20% FCS.

this is like the sort of stuff my flatmate tries to get me to read when she's wanting her essay's checked... i'm checking for what exactly?

[samsonite]

i take it all back. anyone who can get through that is a true legend. all i get at uni is "so if i mix cyan and magenta, what do i get" and that sort of seemingly inconsequential nonsense

[cj]

Thanks guys, I got two coppies in the end, it was too late for the RSL as I'd been in a prac all day, I don't read the materials and methods bit tho, just progressed onto reading the results aswell as abstract and discussion. Nailest, fortunately Armitage is on sabatical so we have some other guy and they've got a bit excited about discussions and presentations recently (probs to prepare us for going into the city!!)